FAST 2.0 Bst DNA Polymerase

Features/Applications:

FAST 2.0 Bst DNA is an extraordinary candidate for isothermal amplifications:

-        DNA strand displacement amplification (SDA) 

-        Cross priming amplification (CPA) 

-        Rolling-circle amplification (RCA) 

-        Loop-mediated isothermal amplification of DNA
         (LAMP) 

-        Reverse transcription isothermal multiple-self-
          matching-initiated amplification (RT-ISMA)

-        Polymerase chain displacement reaction (PCDR) 

-        Sequencing of very low amounts of DNA template

Description:

FAST 2.0 Bst Polymerase (genetically improved Bacillus stearothermophilus cloned to E. Coli) for next generation of isothermal DNA amplification is has been developed recently.

The Polymerase allows the detection within 5 to 10 minutes and thus it is 2 to 3 times quicker than other Bst DNA Polymerases.

The optimized FAST 2.0 Bst Polymerase amplification results can be compared with about 28-31 cycles in a standard PCR-Cycler Platform. The FAST 2.0 Bst Polymerase offers high strand displacement capability

Limitation:

Not recommended for multiplex amplification

Method of detection:

We recommend to use the fluorescent DNA stain EvaGreen to add in the reaction Mix or to use the ready to use  FAST  Mastermix 2.0 Bst with Evagreen Cat.-No. S650 or S660 (Mastermix 2.0 Bst with EvaGreen and ROX)

Content:

8-10 U/µl Fast 2.0 Bst DNA Polymerase, 10 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1 % Triton X-100, 50 %  Glycerol, pH 7.5 @ RT;

- Separate Tube of MgSO4

- Reaction buffer (10X): 200 mM Tris-HCl pH 8.8, KCl, 100 mM (NH4)2SO4, 60 mM MgSO4, enhancer, stabilizer

Concentration: 8 U / µl

Storage condition: @ -20°C

Shipping: on blue ice 

Protocol for 50 µl reaction volume