Features:
- All components like: Taq polymerase, dNTP's, reaction buffers,
enhancers and stabilizers are lyophilized (optional SybrGreen)
- Transportation and storage at room-temperature
- simple, and fast setting up procedure for high yield and repeatable
results
Description:
Maximo Dry Master-Mix is optimized and ready-to-use mixture of all components for a successful PCR. Only your primers and your DNA Template
has to be added to the 20 µl assay.
Maximo Dry Master-Mix contains a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-stranded specific 5´→3´
exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa.
List of components:
12x8 lyophilized PCR-Tubes or PCR Plates (0,2ml) "flat cap",
Unit definition:
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74°C
Quality control:
- PCR with various templates – genomic DNA, Phage Lambda DNA
- 2 kb DNA amplification from 50 ng DNA
- batch variation and level of bacterial DNA contamination
Transportation: at room temperature
Storage: store at room-temperature in an aluminium coated bag or on another dry place; humidity < 65 % when sealing
is opened
Shelf live: 12 months
Quality control:
- PCR with various templates – genomic DNA, Phage Lambda DNA
- 2 kb DNA amplification from 50 ng DNA
- batch variation and level of bacterial DNA contamination
Components |
Volume per reaction |
1 Tube of Maximo Dry Mastermix |
|
Forward Primer: |
0,2-1 µM – 0,4-2µl / 10 µM |
Reverse Primer |
0,2-1 µM – 0,4-2µl / 10 µM |
Template DNA |
1-50 ng |
Sterile dest. Water (molecular grade) |
up to 20 µl total reaction volume |
Note:
- vortex all solutions and spin down carefully before using
The lyophilized Mastermix is also available with HotStart Taq Polymerase, for dual labelled probes or with SybrGreen as fluorescent dye in several reaction chambers of your choice:
96-well PCR plates:
full skirt: high and low profile
half skirt: high and low profile
no skirt: high and low profile
8-tubes strips:
flat cap: high and low profile
PCR Tubes are the most common requested size!
The LYO-Mastermixes for Standard PCR applications are available in many different formats:
96-well PCR plates:
full skirt: high and low profile
half skirt: high and low profile
no skirt: high and low profile
8-tubes strips:
flat cap: high and low profile
After your order we contact you regarding the format you want or you send in advance.
Final price excl. shipping costs3
The LYO-Mastermixes for HOT-Start PCR are available in many different formats:
96-well PCR plates:
full skirt: high and low profile
half skirt: high and low profile
no skirt: high and low profile
8-tubes strips:
flat cap: high and low profile
After your order we contact you regarding the format you want or you send in advance.
Final price excl. shipping costs3
The LYO-Mastermixes SybrGreen as dye are available in many different formats:
96-well PCR plates:
full skirt: high and low profile
half skirt: high and low profile
no skirt: high and low profile
8-tubes strips:
flat cap: high and low profile
After your order we contact you regarding the format you want or you send in advance.
Final price excl. shipping costs3
The LYO-Mastermixes for PCR with dual labelled probes are available in different formats:
96-well PCR plates:
full skirt: high and low profile
half skirt: high and low profile
no skirt: high and low profile
8-tubes strips:
flat cap: high and low profile
After your order we contact you regarding the format you want or you send in advance.
Final price excl. shipping costs3
Dry Taq DNA. Pol DNA PreMix ready-to-use und gefriergetrocknet