50 kbp high-range DNA ladder

Features: GeneON's 50 kb DNA Marker is composed of 17 individual DNA fragments which are re-dissolved in storage buffer.

Description/Preparation: The 50 kb DNA Ladder has a number of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 17 bands suitable for use as molecular weight standards for agarose gel electrophoresis.  The 50 kb DNA Ladder was designed for qualitative and approximately quantification of DNA mass. There are no unspecific bands besides the fragments. The 3000 bp fragment is used a reference band.

Usage: max. 1 µg/lane

Concentration:  200 µg/ml - 1250 µl

Storage Buffer: 10 mM Tris-HCl (pH 8.0), 1.0 mM EDTA, 50mM NaCl 

Tracking Dye: 6X DNA Loading dye as separate vial included (0,01 % Bromophenol blue, 0,25 % M EDTA, 50 % Glycerol)

Number of bands: 17 fragments

Loading: 

1. In the case of standard gel electrophoresis we recommend to use 0.6% agarose gel in TAE Buffer, voltage - 120 V, running time - 4 hours. 

We recommend loading 2-4 μl (0.4-0.8 μg) of the DNA Ladder per line (plus 1/10 v/v of a loading dye).

2. In the case of pulse field gel electrophoresis we recommend to use 0.7-0.8% agarose TAE gel. The voltage and running time depend on the type of the chamber.

Transportation: shipped on blue ice or room temperature

Storage: at -20°C for 24 months.

DNA Leiter 50 kbp "large range"