Activity in SEBuffers:

SEBuffer B       10 -  25%

SEBuffer G       10 -  25%

SEBuffer O       10 -  25%

SEBuffer W      25 -  50%

SEBuffer Y              100%

SEBuffer ROSE        50%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.

Store at: -20°C.

Ligation: After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Reagents supplied with enzyme: 10 X SE-buffer Y

Methylation sensitivity: Blocked by

5`-GGNN(5mC)C-3`/3-C(5mC)NNGG-5` or

5`-GGNN(5mC)C-3`/3-CCNNGG-5` methylation

Inactivation: 20 minutes under 65°C