Activity in SEBuffers:

SEBuffer B          0 - 10%

SEBuffer G        75 -100%

SEBuffer O        50 - 75%

SEBuffer W       25 - 50%

SEBuffer Y              100%

SEBuffer ROSE       100%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 65°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.

Store: at -20°C.

Ligation: After 10-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C.

Reagents supplied with Enzyme: 10 X SE-buffer Y

Methylation sensitivity: not tested

Inactivation: 20 minutes under 80°C