PstN I - CAGNNN↑CTG - GTC↓NNNGAC

Activity in SEBuffers:

SEBuffer B        25 -  50%

SEBuffer G       50 -  75%

SEBuffer O       10 -  25%

SEBuffer W       25 -  50%

SEBuffer Y               100%

SEBuffer ROSE       100%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol;

Store at: -20°C.

Ligation: After 5-fold overdigestion with enzyme > 95% of Lambda DNA fragments can be ligated with T4 DNA Ligase and recut.

Non-specific hydrolisis: No nonspecific

activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.

Reagents supplied with enzyme: 10 X SE-buffer Y

Methylation sensitivity: not tested

Inactivation: 20 minutes under 65°C

Notes: When using a buffer other than the optimal (supplied) SE-buffer, it may be necessary to add more enzyme to achieve complete digestion.