Activity in SEBuffers:

SEBuffer B         50 -  50%

SEBuffer G         50 -  75%

SEBuffer O                100%

SEBuffer W        75 - 100%

SEBuffer Y         10 -  25%

SEBuffer ROSE          50%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; and 50% glycerol.

Store at: -20°C.

Ligation: After 10-fold overdigestion with enzyme >90% of the DNA fragments can be ligated and recut

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C

Reagents supplied with enzyme: 10 X SE-buffer O

Methylation sensitivity: not tested

Inactivation: 20 minutes under 65°C