Activity in SEBuffers:

SEBuffer B              100%

SEBuffer G       50 -  75%

SEBuffer O         0 -  1 0%

SEBuffer W        0  -  10%

SEBuffer Y        75 - 100%

SEBuffer ROSE         50%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA and 50% glycerol;

Store at: -20°C.

Ligation: After 3-fold overdigestion with enzyme 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and 95% of these can be recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 0.5 u.a. of enzyme for 16 hours at 37°C.

Reagents supplied with enzyme: 10 X SE-buffer B

Methylation sensitivity: Not tested

Inactivation: 20 minutes under 65°C