Activity in SEBuffers:

SEBuffer B           25 - 50%

SEBuffer G          25 - 50%

SEBuffer O          75 -100%

SEBuffer W                100%

SEBuffer Y           25 - 50%

SEBuffer ROSE         100%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 60°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; and 50% glycerol

Store: at -20°C

Ligation: After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60°C.

Reagents Supplied with Enzyme: 10 X SE-buffer W

Methylation sensitivity: not tested

Inactivation: 20 minutes under 80°C

Notes: High enzyme concentration may result in star activity. At 37°C activity is 50% from maximum.

The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,5.