Activity in SEBuffers:

SEBuffer B         0 - 10%

SEBuffer G       10 - 25%

SEBuffer O       25 - 50%

SEBuffer W            100%

SEBuffer Y       75 -100%

SEBuffer ROSE     100%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 55°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.

Store: at -20°C.

Ligation: After 20-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of DNA with 40 u.a. of enzyme for 16 hours at 55°C.

Reagents supplied with Enzyme: 10 X SE-buffer W, BSA

Methylation sensitivity: not tested

Inactivation: 20 minutes under 80°C

Notes: Do not use BSA for long incubation . To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.