Activity in SEBuffers:

SEBuffer B       75 - 100%

SEBuffer G       75 - 100%

SEBuffer O       25 -  50%

SEBuffer W       10 -  25%

SEBuffer Y               100%

SEBuffer ROSE       100%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol.

Store at: -20°C.

Ligation: After 2-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C.

Reagents supplied with enzyme: 10 X SE-buffer Y, BSA

Methylation sensitivity: not tested

Inactivation: 20 minutes under 65°C

Notes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.