BstBA I - YAC↑GTR - RTG↓CAY

Activity in SEBuffers:

SEBuffer B       25 - 50%

SEBuffer G       25 - 50%

SEBuffer O       75 -100%

SEBuffer W             100%

SEBuffer Y        25 - 50%

SEBuffer ROSE       50%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 65°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.

Store at: -20°C.

Ligation: After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C.

Reagents supplied with enzyme: 10 X SE-buffer W, BSA

Methylation sensitivity: Blocked by CG methylation.

Inactivation: 20 minutes under 80°C

Notes: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.