Gsa I - CCCAG↑C - G↓GGTCG

Activity in SEBuffers:

SEBuffer B         10 - 25%

SEBuffer G         25 - 50%

SEBuffer O         75 -100%

SEBuffer W              100%

SEBuffer Y        75 -100%

SEBuffer ROSE      100%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 70°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.

Store at: -20°C.

Ligation: After 20-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 70°C.

Reagents Supplied with Enzyme: 10 X SE-buffer W, BSA

Methylation sensitivity: not tested

Inactivation: 20 minutes no

Notes: Do not use BSA for long incubation. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.