ready to use 2x PCR Master mix with SybrGreen and Blue dye. 2 x 1 ml

Components and Mixture

- 2X BIO-Star Universal-Flex qPCR-Mastermix SYBR Blue contains (2x1 ml):

The master mix is a ready-to-use solution including Hot Start Taq DNA polymerase, dNTPs (dATP, dCTP, dGTP, dTTP) in an optimized PCR buffer system, SybrGreen I, novel passive ROX alike as reference dye and blue inert-dye which does not interfere the PCR-reaction in a real-time Cycler.

Features:

- A novel passive ROX alike reference dye. BIO-Star Universal-Flex Mastermix is

  compatible with all common PCR-Platforms (no ROX, low ROX of high ROX, see Combability list)

- Enzyme with hot start capability increases reaction specificity and sensitivity

- Hot Start Taq DNA polymerase with monoclonal antibodies

- High selectivity and reaction yield

- The mix is featured by a non-fluorescent blue dye for easy pipetting and visual control

 Applications:

- Real-time PCR with intercalating dye SYBR Green I (valid for all ROX concentrations)

- Conventional PCR (cDNA, plasmid DNA, Lambda DNA)

- High-throughput PCR

Description:

The Mastermix contains all components all reagents required for qPCR (except template and primers) The SYBR Green I dye binds to double-stranded DNA formed during real-time PCR. At the same time, the product contains a special ROX Passive Reference Dye, which is suitable for use in all qPCR instruments, and there is no need to adjust the ROX concentration on different instruments.

Taqs: Luna, NEB,