Activity in SEBuffers:

SEBuffer B         50 -  75%

SEBuffer G        75 - 100%

SEBuffer O               100%

SEBuffer W       75 - 100%

SEBuffer Y         50 -  75%

SEBuffer ROSE          50%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol;

Store at: -20°C.

Ligation: After 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Reagents supplied with enzyme: 10 X SE-buffer O

Methylation sensitivity: Not blocked by ACmATGT methylation. Blocked by mACATGT methylation.

Inactivation: 20 minutes under 65°C

Notes: The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,13.