Description: 

The presence of genomic DNA in RNA preparations is often a significant problem for downstream applications. This contamination often leads to false-positive signals and misrepresentation of gene expression levels.

gDNA Removal Kit from GeneON removes gDNA from RNA prior to reverse transcription.

The Removal Kit is based on the recombinant heat labile dsDNase, which is irreversibly inactivated at moderate temperatures.

After that inactivation step which is gentle enough to preserve both quality and quantity of all present RNA.

Reverse Transcription can be performed in the same tube, thereby minimizing pipetting steps and reducing hands-on time.

Shipping: shipped on blue ice

Storage: store at -20°C, avoid frequent freeze / thaw cycles

Shelf Life: 16 months

Complete removal of genomic DNA from RNA preps

Most techniques used for RNA isolation yield RNA with significant amounts of contaminating genomic DNA (gDNA), potentially resulting in false and inaccurate mRNA quantification. This is particularly a problem in the quantification of low-copy transcripts or small samples. The gDNA Removal Kit efficiently removes gDNA from RNA preps to levels below the detection limit of RT-qPCR (Figure).

Content:

gDNA Remover reagent

(red cap) - Heat-inactivatable dsDNase

10x Reaction Buffer

(green cap)

Protocol

Perform the assay set-up as following:

Incubation time:

- 10 min @ 37°C or 20 min at 25°C

-   5 min @ 58°C for inactivation of the enzyme

The reverse transcription reagents can be directly added to the tube containing the purified RNA sample, after completion of DNase treatment,