EXO I - SAP clean up kit
Alkaline Phosphatase / Exonuclease mix for clean up of PCR products
Description:
The Kit contains two hydrolytic enzymes, recombinant Shrimp Alkaline Phosphatase (rSAP) and Exonuclease I (Exo I).
The combination of these enzymes ensures complete dephosphorylation of dNTPs and degradation of residual primers, enabling downstream applications, such as sequencing, genotyping, cloning or SNP analysis.
The reagents are active in commonly used PCR buffers and eliminates the need for additional buffer exchange.
Content: SAP-Exo Mix (red cap)
Shipping: shipped on blue ice
Storage Conditions: store at -20°C
Notes: avoid multiple freeze / thaw cycles
Shelf Life: at least 18 months
Stability tests / Quality control / Comparison
Applications:
SAP-Exo Kit is a quick, easy and reliable enzymatic cleanup reagent for PCR product clean up.
• Removes excess primers and dNTPs
• Fast 20 minute protocol
• Add directly to PCR product
• 100% Sample Recovery
• Scalable for different reaction sizes
• No interference on downstream applications
• Easy to automate
Protocol:
-Add 2µl of SAP-Exo mix directly to 5µl PCR product (or scale up, i.g. 4µl
SAP-Exo mix to 10µl PCR product) and mix well
-Incubate for 10 min at 37°C
-Inactivate for 10 min at 80°C
PCR products are ready for downstream applications without any need for further processing.
SAP-Exo Kit is a quick, easy and reliable enzymatic cleanup reagent for PCR product clean up.
* availibility of sample size may be limited
Final price3
- verfügbar / avaílable
Free sample request
Dear customer, GeneON likes to send free samples to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .
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Deutsche Beschreibung
EXO I - SAP clean up kit
Datasheet EXO I - SAP clean up kit
• Removes excess primers and dNTPs
• Fast 20 minute protocol
• Add directly to PCR product
• 100% Sample Recovery
• Scalable for different reaction sizes
• No interference on downstream applications
• Easy to automate
Material Safety Datasheet
References / Protocols / Notes / Recomendations / Tests
Contact
GeneOn GmbH
Paracelsusstrasse 10
67071 Ludwigshafen am Rhein
Deutschland / Germany
Phone: +49-621-5720 864
Fax: +49-621-5724 462
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- DNA/RNA Extraction
- Real-time PCR
- RT-qPCR - Master Mixes
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- RT-PCR - Reverse Transcription
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GENEON GmbH - Germany - Paracelsusstrasse 10 - 67071 Ludwigshafen - www.geneon.net - info(at)geneon.net - Tel: 0621-5720864