Xma I - C↑CCGGG - GGGCC↓C

Activity in SEBuffers:

SEBuffer B       75 - 100%

SEBuffer G       50 -  75%

SEBuffer O         0 -  10%

SEBuffer W         0 -  10%

SEBuffer Y              100%

SEBuffer ROSE        50%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 20 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol.

Store at: -20°C.

Ligation: After 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated. Of these 90% can be recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 3 u.a. of enzyme for 16 hours at 37°C

Reagents supplied with enzyme: 10 X SE-buffer Y

Methylation sensitivity: not tested

Inactivation: 20 minutes under 65°C