Activity in SEBuffers:

SEBuffer B       75 -100%

SEBuffer G       75 -100%

SEBuffer O       25 - 50%

SEBuffer W       50 - 75%

SEBuffer Y             100%

SEBuffer ROSE       30%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 37°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol;

Store: at -20°C.

Ligation: After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C

Reagents supplied with Enzyme: 10 X SE-buffer Y, BSA

Methylation sensitivity: not tested

Inactivation: 20 minutes under 80°C

Notes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.  Do not use BSA for long incubation.