Activity in SEBuffers:

SEBuffer B       75 -100%

SEBuffer G             100%

SEBuffer O        50-100%

SEBuffer W      75 -100%

SEBuffer Y       75-100%

SEBuffer ROSE      100%

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

Optimal temperature: 55°C

Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol.

Store: at -20°C.

Ligation: After 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 55°C.

Reagents supplied with Enzyme: 10 X SE-buffer G

Methylation sensitivity: not tested

Inactivation: 20 minutes under 80°C

Notes: at 37°C activity is 10% from maximum.