One.Step RT-PCR Probe Kit
The MaximoOne.Step RT-qPCR Kit is designed for quantitative real-time analyses of RNA templates using Dual Labeled Fluorescent Probes
Features:
- The kit contains all reagents required for RT-qPCR in a set to ensure fast and easy preparation with a minimum of pipetting steps. Just add template, primers and the dual labeled fluorescent probe.
- The One.Step RT-PCR Kit is highly sensitive: less than 10 pg to 100 ng total RNA or < 1 pg to 20 ng poly(A) RNA (mRNA) can be detected when using highly expressed transcripts
The enzyme mix is based on a new formulated reverse transcriptase, a Hot-Start Polymerase and RNase Inhibitor. The set is providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments. The reaction buffer includes extrapure dNTPs and reaction enhancers. The basis is a Real-time PCR with dual labeled probes and multiplexing capacity.
Principal:
In the RT-step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. The hot-start polymerase activity is blocked.
In the first cycle of the PCR step the Hot-Start DNA polymerase activity is switched on and it synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template.
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The One.step Realtime RT-PCR Master Mix from GeneON offers a tremendous convenience when applied to analysis of targets from multiple samples of RNA and minimizes the risk of contaminations. |
Platforms: The Kit is suitable for all block-based Thermocycler. Stringent Quality Tests on ABI StepOne plus PCR Cycler
Components of Maximo.OneStep RT-qPCR (for probes):
Enzyme-mix: HotStart Taq Polymerase, Reverse Transcriptase, RNase Inhibitor and enhancers, Reaction buffer, extra pure dNTPs
RNase-free water
Storage:
@ -20°C, avoid frequent thawing and freezing
Transport:
the product will be shipped with "blue ice"
For optimal results it is recommended to make an individually optimization for each RNA / primer pair.
Stability tests / Quality control / Comparison
Manufactured and quality-controlled in accordance with ISO 9001:2000
Components: Enzyme-mix: HotStart Taq Polymerase, Reverse Transcriptase, RNase Inhibitor and enhancers, Reaction buffer, extra pure dNTPsRNase-free water
Protocols / Manuals
The datasheet includes detailed protocols for:
1.) RT-PCR assay without sample denaturation (as a standard RNA/primer combinations)
2.) RT-PCR assay with sample denaturation (RNA/primer with a high degree of secondary
structure)
2.b) Denaturation and primer annealing
2.c) Preparation of the RT-PCR Mix
3.) Reverse Transcription and thermal cycling
The MaximoOne.Step RT-qPCR Kit is designed for quantitative real-time analyses of RNA templates using Dual Labeled Fluorescent Probes
* availibility of sample size may be limited
Final price excl. shipping costs3
- verfügbar / avaílable
- 1 - 3 days for delivery / 1 - 3 Tage Lieferzeit1
Deutsche Beschreibung
One.Step RT-PCR Probe Master Mix
Datasheet One.Step RT-PCR Probe Master Mix
Material Safety Datasheet
References / Protocols / Notes / Recomendations / Tests
Contact
GeneOn GmbH
Paracelsusstrasse 10
67071 Ludwigshafen am Rhein
Deutschland / Germany
Phone: +49-621-5720 864
Fax: +49-621-5724 462
Shop-Terms
AGB / Businesss Condtitions / Freight Costs / Frachtkosten für Deutschland
See Categories
- DNA/RNA Extraction
- Real-time PCR
- RT-qPCR - Master Mixes
- PCR / DNA Amplification
- RT-PCR - Reverse Transcription
- Nucleotides, dNTPs, Primers
- Marker / Gel Staining.
- Enzymes and Fine chemicals
- Fluorescent Dyes / supplements
- DNA/Ladders/Standards
- PCR-96 plates and tools, Tips, Tubes, Filtration
- Restriction Endonucleases
- Devices and Tools
News & Informations
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GENEON GmbH - Germany - Paracelsusstrasse 10 - 67071 Ludwigshafen - www.geneon.net - info(at)geneon.net - Tel: 0621-5720864